Nature

Structural modeling of protein–RNA complexes using crosslinking of segmentally isotope-labeled RNA and MS/MS

Figure 1: CLIR-MS/MS analysis of crosslinked PTBP1-peptides. Figure 2: CLIR-MS/MS mapping of PTBP1 on EMCV-IRES domains D–F. In summary, CLIR-MS/MS revealed the precise structural arrangement of PTBP1 ...
Nature

Incorporation of isotopic, fluorescent, and heavy-atom-modified nucleotides into RNAs by position-selective labeling of RNA

The overwhelming majority of RNA structures at atomic resolution in the Protein Data Bank were solved by NMR or X-ray crystallography. Both methods can be greatly facilitated by the availability of ...
technologynetworks

Strand-specific Transcriptome Profiling with Directly Labeled RNA

With lower manufacturing cost, high spot density, and flexible probe design, genomic tiling microarrays are ideal for comprehensive transcriptome studies. Typically, transcriptome profiling using ...